Molecular Investigation of Enterotoxigenic Staphylococcus aureus Isolates from Restaurant Staff

Background: Intoxication by heat-resistant Staphylococcus aureus enterotoxins (SEs), particularly SEA and SEE, is one of the most common causes food poisoning in world. Objectives: This study aimed to identify enterotoxin-encoding genes (see, sed, sec, seb, sea) S. isolates from different restaurant employees. Methods: Ninety-one samples hands noses employees several restaurants Golestan Province (North Iran) were examined for detection strains phenotypic microbiological genotyping methods. After determining susceptibility ten antibiotics Kirby-Bauer method, enterotoxin-producing detected using polymerase chain reaction method. Results: was 29 (31.9%) samples. The frequency highest waiters (65.5%) with age range 34 - 49 years (41.4%) nasal (55.1%). lowest rates observed against linezolid (100%) cefoxitin (17.24%). Of 19 methicillin-resistant strains, 89.47% identified as multi-drug resistant 10.52% extensively-drug resistant. isolates, 24.13% enterotoxigenic, belonging (57.1%). Conclusions: In this study, we isolated resistant, enterotoxigenic employees, which can have important public health implications. Therefore, it recommended closely monitor health, sanitation, hygiene people involved preparation reduce spread bacteria chain.